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Int J Mol Sci. 2015 Sep 9;16(9):21681-94. doi: 10.3390/ijms160921681.

A New HPLC-MS Method for Measuring Maslinic Acid and Oleanolic Acid in HT29 and HepG2 Human Cancer Cells.

Author information

1
Biochemistry and Molecular Biology Section, Department of Experimental Biology, University of Jaén, Campus Las Lagunillas, E-23071 Jaén, Spain. jperagon@ujaen.es.
2
Department of Biochemistry and Molecular Biology I, Faculty of Sciences, University of Granada, Campus Fuentenueva, E-18001 Granada, Spain. evaevae@ugr.es.
3
Biochemistry and Molecular Biology Section, Department of Experimental Biology, University of Jaén, Campus Las Lagunillas, E-23071 Jaén, Spain. mebeverina@gmail.com.
4
Department of Biochemistry and Molecular Biology I, Faculty of Sciences, University of Granada, Campus Fuentenueva, E-18001 Granada, Spain. ferjes@ugr.es.
5
Department of Biochemistry and Molecular Biology I, Faculty of Sciences, University of Granada, Campus Fuentenueva, E-18001 Granada, Spain. jlcara@ugr.es.

Abstract

Maslinic acid (MA) and oleanolic acid (OA), the main triterpenic acids present in olive, have important properties for health and disease prevention. MA selectively inhibits cell proliferation of the HT29 human colon-cancer cell line by inducing selective apoptosis. For measuring the MA and OA concentration inside the cell and in the culture medium, a new HPLC-MS procedure has been developed. With this method, a determination of the amount of MA and OA incorporated into HT29 and HepG2 human cancer-cell lines incubated with different concentrations of MA corresponding to 50% growth inhibitory concentration (IC50), IC50/2, IC50/4, and IC50/8 has been made. The results demonstrate that this method is appropriate for determining the MA and OA concentration in different types of cultured cells and reveals the specific dynamics of entry of MA into HT29 and HepG2 cells.

KEYWORDS:

HPLC-MS; HT29; HepG2; maslinic acid; oleanolic acid

PMID:
26370984
PMCID:
PMC4613274
DOI:
10.3390/ijms160921681
[Indexed for MEDLINE]
Free PMC Article

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