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FASEB J. 2016 Jan;30(1):252-61. doi: 10.1096/fj.15-276840. Epub 2015 Sep 14.

The microbiota shifts the iron sensing of intestinal cells.

Author information

1
*INSERM U1016, Institut Cochin, Paris, France; Centre National de la Recherche Scientifique, Unité Mixte de Recherche 8104, Paris, France; Université Paris Descartes and Université Paris Diderot, site Bichat, Sorbonne Paris Cité, Paris, France; Laboratory of Excellence GR-Ex, Paris, France; Institut National de la Recherche Agronomique, AgroParisTech, and Commensal and Probiotics-Host Interactions Laboratory, Unité Mixte de Recherche 1319, Microbiologie de l'Alimentation au Service de la Santé, Jouy-en-Josas, France; **INSERM Unité 1043-Centre de Physiopathologie de Toulouse Purpan and Université de Toulouse, Université Paul Sabatier, Centre de Physiopathologie de Toulouse Purpan, Toulouse, France; Centre National de la Recherche Scientifique Unité 5282, Toulouse, France; and INSERM Unité 1149, Centre de Recherches sur l'Inflammation, Paris, France.
2
*INSERM U1016, Institut Cochin, Paris, France; Centre National de la Recherche Scientifique, Unité Mixte de Recherche 8104, Paris, France; Université Paris Descartes and Université Paris Diderot, site Bichat, Sorbonne Paris Cité, Paris, France; Laboratory of Excellence GR-Ex, Paris, France; Institut National de la Recherche Agronomique, AgroParisTech, and Commensal and Probiotics-Host Interactions Laboratory, Unité Mixte de Recherche 1319, Microbiologie de l'Alimentation au Service de la Santé, Jouy-en-Josas, France; **INSERM Unité 1043-Centre de Physiopathologie de Toulouse Purpan and Université de Toulouse, Université Paul Sabatier, Centre de Physiopathologie de Toulouse Purpan, Toulouse, France; Centre National de la Recherche Scientifique Unité 5282, Toulouse, France; and INSERM Unité 1149, Centre de Recherches sur l'Inflammation, Paris, France muriel.thomas@jouy.inra.fr gael.nicolas@inserm.fr.

Abstract

The amount of iron in the diet directly influences the composition of the microbiota. Inversely, the effects of the microbiota on iron homeostasis have been little studied. So, we investigate whether the microbiota itself may alter host iron sensing. Duodenal cytochrome b and divalent metal transporter 1, involved in apical iron uptake, are 8- and 10-fold, respectively, more abundant in the duodenum of germ-free (GF) mice than in mice colonized with a microbiota. In contrast, the luminal exporter ferroportin is 2-fold less abundant in GF. The overall signature of microbiota on iron-related proteins is similar in the colon. The colonization does not modify systemic parameters as plasma transferrin saturation (20%), plasma ferritin (150 ng/L), and liver (85 µg/g) iron load. Commensal organisms (Bacteroides thetaiotaomicron VPI-5482 and Faecalibacterium prausnitzii A2-165) and a probiotic strain (Streptococcus thermophilus LMD-9) led to up to 12-fold induction of ferritin in colon. Our data suggest that the intestinal cells of GF mice are depleted of iron and that following colonization, the epithelial cells favor iron storage. This study is the first to demonstrate that gut microbes induce a specific iron-related protein signature, highlighting new aspects of the crosstalk between the microbiota and the intestinal epithelium.

KEYWORDS:

ferroportin; iron storage; iron transporters; microbes

PMID:
26370847
DOI:
10.1096/fj.15-276840
[Indexed for MEDLINE]

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