Format

Send to

Choose Destination
J Proteomics. 2016 Jan 1;130:26-32. doi: 10.1016/j.jprot.2015.08.024. Epub 2015 Sep 11.

Urine proteome analysis in Dent's disease shows high selective changes potentially involved in chronic renal damage.

Author information

1
Laboratory on Physiopathology of Uremia, Istituto Giannina Gaslini, Genova, Italy.
2
Laboratory of Histomorphology and Molecular Biology of the Kidney, Division of Nephrology, University of Padova, Padova, Italy.
3
Nephrology, Dialysis, Transplantation Unit, Azienda Ospedaliera-University of Padova, Padova, Italy.
4
Proteomics and Mass Spectrometry Laboratory, Istituto per il Sistema Produzione Animale in Ambiente Mediterraneo (ISPAAM) National Research Council (CNR), Napoli, Italy.
5
Laboratory of Mass Spectrometry - Core Facility, Istituto Giannina Gaslini, Genova, Italy.
6
Nephrology, Dialysis and Transplantation Unit, Istituto Giannina Gaslini, Genova, Italy.
7
Nephrology, Dialysis and Transplantation Unit, Istituto Giannina Gaslini, Genova, Italy; Laboratory on Physiopathology of Uremia, Istituto Giannina Gaslini, Genova, Italy. Electronic address: GMarcoGhiggeri@ospedale-gaslini.ge.it.

Abstract

Definition of the urinary protein composition would represent a potential tool for diagnosis in many clinical conditions. The use of new proteomic technologies allows detection of genetic and post-trasductional variants that increase sensitivity of the approach but complicates comparison within a heterogeneous patient population. Overall, this limits research of urinary biomarkers. Studying monogenic diseases are useful models to address this issue since genetic variability is reduced among first- and second-degree relatives of the same family. We applied this concept to Dent's disease, a monogenic condition characterised by low-molecular-weight proteinuria that is inherited following an X-linked trait. Results are presented here on a combined proteomic approach (LC-mass spectrometry, Western blot and zymograms for proteases and inhibitors) to characterise urine proteins in a large family (18 members, 6 hemizygous patients, 6 carrier females, and 6 normals) with Dent's diseases due to the 1070G>T mutation of the CLCN5. Gene ontology analysis on more than 1000 proteins showed that several clusters of proteins characterised urine of affected patients compared to carrier females and normal subjects: proteins involved in extracellular matrix remodelling were the major group. Specific analysis on metalloproteases and their inhibitors underscored unexpected mechanisms potentially involved in renal fibrosis.

BIOLOGICAL SIGNIFICANCE:

Studying with new-generation techniques for proteomic analysis of the members of a large family with Dent's disease sharing the same molecular defect allowed highly repetitive results that justify conclusions. Identification in urine of proteins actively involved in interstitial matrix remodelling poses the question of active anti-fibrotic drugs in Dent's patients.

KEYWORDS:

CLCN5 gene; Chronic renal failure; Dent's disease; Fibrosis; Metalloproteases; TIMPs

PMID:
26370164
DOI:
10.1016/j.jprot.2015.08.024
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center