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J Cell Biochem. 2016 Apr;117(4):938-45. doi: 10.1002/jcb.25378. Epub 2015 Sep 22.

Commercially Available Preparations of Recombinant Wnt3a Contain Non-Wnt Related Activities Which May Activate TGF-β Signaling.

Author information

1
Ludwig Institute for Cancer Research, Science for Life Laboratory, Uppsala University, Box 595 Biomedical Center, SE-751 24, Uppsala, Sweden.
2
Department of Medical Biochemistry and Microbiology, Science for Life Laboratory, Uppsala University, Box 582 Biomedical Center, SE-751 23, Uppsala, Sweden.

Abstract

The Wnt ligands are a family of secreted signaling proteins which play key roles in a number of cellular processes under physiological and pathological conditions. Wnts bind to their membrane receptors and initiate a signaling cascade which leads to the nuclear localization and transcriptional activity of β-catenin. The development of purified recombinant Wnt ligands has greatly aided in our understanding of Wnt signaling and its functions in development and disease. In the current study, we identified non-Wnt related signaling activities which were present in commercially available preparations of recombinant Wnt3a. Specifically, we found that treatment of cultured fibroblasts with recombinant Wnt3a induced immediate activation of TGF-β and BMP signaling and this activity appeared to be independent of the Wnt ligand itself. Therefore, while purified recombinant Wnt ligands continue to be a useful tool for studying this signaling pathway, one must exercise a degree of caution when analyzing the results of experiments that utilize purified recombinant Wnt ligands.

KEYWORDS:

BMP; FIBROBLAST; SMAD; TGF-β; Wnt; Wnt3a

PMID:
26369756
DOI:
10.1002/jcb.25378
[Indexed for MEDLINE]
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