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PLoS One. 2015 Sep 14;10(9):e0136770. doi: 10.1371/journal.pone.0136770. eCollection 2015.

Mature Erythrocytes of Iguana iguana (Squamata, Iguanidae) Possess Functional Mitochondria.

Author information

1
Dipartimento di Biologia, Università Tor Vergata, Roma, Italia.
2
Dipartimento di Biologia, Università Tor Vergata, Roma, Italia; IRCCS Fondazione Santa Lucia, Roma, Italia.
3
IRCCS Fondazione Santa Lucia, Roma, Italia; Istituto Telethon Dulbecco, Istituto Veneto di Medicina Molecolare, Padova, Italia.
4
Dipartimento di Biologia, Università Tor Vergata, Roma, Italia; Cell Stress and Survival Unit, Danish Cancer Society Research Center, Copenhagen, Denmark.

Abstract

Electron microscopy analyses of Iguana iguana blood preparations revealed the presence of mitochondria within erythrocytes with well-structured cristae. Fluorescence microscopy analyses upon incubation with phalloidin-FITC, Hoechst 33342 and mitochondrial transmembrane potential (Δψm)-sensitive probe MitoTracker Red indicated that mitochondria i) widely occur in erythrocytes, ii) are polarized, and iii) seem to be preferentially confined at a "perinuclear" region, as confirmed by electron microscopy. The analysis of NADH-dependent oxygen consumption showed that red blood cells retain the capability to consume oxygen, thereby providing compelling evidence that mitochondria of Iguana erythrocytes are functional and capable to perform oxidative phosphorylation.

PMID:
26367118
PMCID:
PMC4569276
DOI:
10.1371/journal.pone.0136770
[Indexed for MEDLINE]
Free PMC Article

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