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Clin Cosmet Investig Dermatol. 2015 Sep 2;8:463-70. doi: 10.2147/CCID.S84903. eCollection 2015.

The role of vitamin C in pushing back the boundaries of skin aging: an ultrasonographic approach.

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Clinic of Dermatology and Allergology, University Clinic Ulm, Ulm, Germany.
Clinic of Dermatology and Venereology, Iuliu HaĊ£ieganu University of Medicine and Pharmacy, Cluj-Napoca, Romania.
Department of Clinical Imaging Ultrasound, Iuliu HaĊ£ieganu University of Medicine and Pharmacy, Cluj-Napoca, Romania.



Imagistic methods stand as modern, non-invasive, and objective means of assessing the impact of topical cutaneous therapies.


This study focuses on the evaluation, by high-frequency ultrasound, of the cutaneous changes induced by topical use of a vitamin C complex at facial level.


A vitamin C-based solution/Placebo moisturizer cream was applied at facial level of 60 healthy female subjects according to a predetermined protocol. Ultrasonographic images (Dermascan C, 20 MHz) were taken from zygomatic level initially, at 40 and 60 days after therapy. The following parameters were assessed for every subject: thickness of the epidermis and dermis (mm), the number of low (LEP), medium (MEP), high echogenic pixels (HEP), and the number of LEP in the upper dermis/lower dermis (LEPs/LEPi).


LEP decreased significantly in all age categories during and after therapy, but especially in the first 2 age intervals, up to the age of 50 (P=0.0001). MEP and HEP, pixel categories that quantify protein synthesis also had an age-dependent evolution in the study, increasing significantly in all age categories but most of all in the first age interval (P=0.002). Our ultrasonographic data suggest that collagen synthesis increased significantly after topical vitamin C therapy, and is responsible for the increase in MEP and HEP and consequent decrease of the LEP.


Our study shows that topically applied vitamin C is highly efficient as a rejuvenation therapy, inducing significant collagen synthesis in all age groups with minimal side effects.


ascorbic acid; collagen synthesis; rejuvenation; ultrasound

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