Format

Send to

Choose Destination
Oral Oncol. 2015 Nov;51(11):998-1003. doi: 10.1016/j.oraloncology.2015.08.002. Epub 2015 Sep 11.

MicroRNA-24 induces cisplatin resistance by targeting PTEN in human tongue squamous cell carcinoma.

Author information

1
Department of Thyroid and Neck Tumor, Tianjin Medical University Cancer Institute and Hospital, Oncology Key Laboratory of Cancer Prevention and Therapy, National Clinical Research Center of Cancer, Tianjin 300060, PR China. Electronic address: xiangqian_zheng@163.com.
2
Department of Anesthesiology, Tianjin Medical University Cancer Institute and Hospital, Oncology Key Laboratory of Cancer Prevention and Therapy, National Clinical Research Center of Cancer, Tianjin 300060, PR China.
3
Department of Thyroid and Neck Tumor, Tianjin Medical University Cancer Institute and Hospital, Oncology Key Laboratory of Cancer Prevention and Therapy, National Clinical Research Center of Cancer, Tianjin 300060, PR China.
4
Department of Lyphoma, Tianjin Medical University Cancer Institute and Hospital, Oncology Key Laboratory of Cancer Prevention and Therapy, National Clinical Research Center of Cancer, Tianjin 300060, PR China.

Abstract

BACKGROUND:

miR-24 is one of the most significantly up-regulated miRNAs in tongue squamous cell carcinoma (TSCC). PTEN plays an important role in the cell survival and cisplatin resistance of multiple cancers. However, it remains unclear what role does function and mechanism of miR-24 and PTEN play in TSCC.

METHODOLOGY/PRINCIPAL FINDINGS:

In this study, miR-24 expression was detected in 79 cases of paired TSCC and normal tissues and 8 TSCC cell lines by real-time PCR and the relevance between miR-24 expression and clinicopathological parameters were analyzed. Further, we demonstrated that deregulation of miR-24 was found to associate with high grade and late stage tumor. In addition, miR-24 induces cell survival and cisplatin resistance through targeting 3'-UTR region of the PTEN, which leads to downregulation of PTEN protein and activation of Akt pro-survival pathway.

CONCLUSIONS/SIGNIFICANCE:

In conclusion, our results demonstrated that deregulation of miR-24 is a recurrent event in human tongue squamous cell carcinoma and associate with tumor progression and that miR-24 induces cell survival and cisplatin resistance primarily through targeting PTEN/Akt pathway. Thus, miR-24 could be important targets for intervention of this malignancy.

KEYWORDS:

Cisplatin resistance; MicroRNA-24; PTEN; Tongue squamous carcinoma

[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center