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Metab Eng. 2015 Nov;32:39-48. doi: 10.1016/j.ymben.2015.09.001. Epub 2015 Sep 11.

Metabolic and process engineering of Clostridium cellulovorans for biofuel production from cellulose.

Author information

1
Department of Chemical and Biomolecular Engineering and Department of Molecular Genetics, The Ohio State University, 151 West Woodruff Avenue, Columbus, OH 43210, USA.
2
Department of Chemical and Biomolecular Engineering and Department of Molecular Genetics, The Ohio State University, 151 West Woodruff Avenue, Columbus, OH 43210, USA. Electronic address: yang.15@osu.edu.

Abstract

Production of cellulosic biofuels has drawn increasing attention. However, currently no microorganism can produce biofuels, particularly butanol, directly from cellulosic biomass efficiently. Here we engineered a cellulolytic bacterium, Clostridium cellulovorans, for n-butanol and ethanol production directly from cellulose by introducing an aldehyde/alcohol dehydrogenase (adhE2), which converts butyryl-CoA to n-butanol and acetyl-CoA to ethanol. The engineered strain was able to produce 1.42 g/L n-butanol and 1.60 g/L ethanol directly from cellulose. Moreover, the addition of methyl viologen as an artificial electron carrier shifted the metabolic flux from acid production to alcohol production, resulting in a high biofuel yield of 0.39 g/g from cellulose, comparable to ethanol yield from corn dextrose by yeast fermentation. This study is the first metabolic engineering of C. cellulovorans for n-butanol and ethanol production directly from cellulose with significant titers and yields, providing a promising consolidated bioprocessing (CBP) platform for biofuel production from cellulosic biomass.

KEYWORDS:

Biofuel; Butanol; Cellulose; Clostridium cellulovorans; Ethanol; Metabolic engineering

PMID:
26365585
DOI:
10.1016/j.ymben.2015.09.001
[Indexed for MEDLINE]

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