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J Ethnopharmacol. 2016 May 13;183:54-58. doi: 10.1016/j.jep.2015.09.004. Epub 2015 Sep 11.

Anti-Helicobacter pylori activity of bioactive components isolated from Hericium erinaceus.

Author information

1
Key Laboratory of Applied Mycological Resources and Utilization, Ministry of Agriculture; National Engineering Research Center of Edible Fungi; Shanghai Key Laboratory of Agricultural Genetics and Breeding; Institute of Edible Fungi, Shanghai Academy of Agricultural Science, SAAS, Shanghai 201106, China.
2
Key Laboratory of Applied Mycological Resources and Utilization, Ministry of Agriculture; National Engineering Research Center of Edible Fungi; Shanghai Key Laboratory of Agricultural Genetics and Breeding; Institute of Edible Fungi, Shanghai Academy of Agricultural Science, SAAS, Shanghai 201106, China; College of Food Science & Technology, Shanghai Ocean University, Shanghai 200090, China.
3
Key Laboratory of Applied Mycological Resources and Utilization, Ministry of Agriculture; National Engineering Research Center of Edible Fungi; Shanghai Key Laboratory of Agricultural Genetics and Breeding; Institute of Edible Fungi, Shanghai Academy of Agricultural Science, SAAS, Shanghai 201106, China. Electronic address: syj0@saas.sh.cn.

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE:

The fungus Hericium erinaceus (Bull.) Pers is used in Chinese traditional medicine to treat symptoms related to gastric ulcers. Different extracts from the fungus were assessed for anti-Helicobacter pylori activity to investigate the antibacterial activity of the ethanol extracts from H. erinaceus and verify the traditional indication of use.

MATERIALS AND METHODS:

The fruiting bodies of H. erinaceus were concentrated with ethanol by HPD-100 macroporous resin and the whole extract was partitioned by petroleum ether and chloroform to afford fractions with using a silica gel column. Several pure compounds of petroleum ether extracts were obtained and analyzed using nuclear magnetic resonance (NMR). The activity of the extracts and fractions towards H. pylori was assessed by the microdilution assay and by the disk diffusion assay in vitro. From the most active fraction, two pure compounds were isolated and identified as the main components with anti-H. pylori activity from the fungus H. erinaceus. The cytotoxicity of these two compounds against the human erythroleu-kemia cell line K562 was also evaluated.

RESULTS:

The crude ethanol extracts from the fungus H. erinaceus were inhibitory to H. pylori. The petroleum ether extracts (PE1s, PE2s) and the chloroform extracts (TEs) demonstrated strong inhibition to H. pylori. The inhibition of H. pylori was observed through an agar dilution test with minimal inhibition concentration (MIC) values from 400μg/mL to 12.5µg/mL. Two pure compounds, 1-(5-chloro-2-hydroxyphenyl)-3-methyl-1-butanone and 2,5-bis(methoxycarbonyl)terephthalic acid were isolated from the petroleum ether fractions and identified using (1)H NMR and (13)C NMR spectra analysis. The MIC value for 1-(5-chloro-2-hydroxyphenyl)-3-methyl-1-butanone was 12.5-50µg/mL and the MIC value for 2,5-bis(methoxycarbonyl)terephthalic acid was 6.25-25µg/mL. Both two compounds showed weak cytotoxicity against K562 with IC50<200mM.

CONCLUSIONS:

This study revealed that the extracts from petroleum ether contribute to the anti-H. pylori activity. The compounds obtained from petroleum ether extracts, 1-(5-chloro-2-hydroxyphenyl)-3-methyl-1-butanone and 2,5-bis(methoxycarbonyl)terephthalic acid, inhibit the growth of H. pylori.

KEYWORDS:

Anti-Helicobacter pylori activity; Ethanol extracts; Hericium erinaceus; Low-molecular-weight bioactive components

PMID:
26364939
DOI:
10.1016/j.jep.2015.09.004
[Indexed for MEDLINE]

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