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J Chromatogr A. 2015 Oct 16;1416:121-8. doi: 10.1016/j.chroma.2015.08.065. Epub 2015 Sep 3.

Determination of biogenic amines in beer and wine by capillary electrophoresis-tandem mass spectrometry.

Author information

1
Agilent Technologies, Alameda Araguaia, 1142, CEP 06455-000 Barueri, SP, Brazil; Departamento de Química Fundamental - Instituto de Química - Universidade de São Paulo, Av. Prof. Lineu Prestes, 748, CEP 05508-000 São Paulo, SP, Brazil. Electronic address: daniela_daniel@agilent.com.
2
Departamento de Química Fundamental - Instituto de Química - Universidade de São Paulo, Av. Prof. Lineu Prestes, 748, CEP 05508-000 São Paulo, SP, Brazil. Electronic address: vagnerbs@iq.usp.br.
3
Departamento de Química Fundamental - Instituto de Química - Universidade de São Paulo, Av. Prof. Lineu Prestes, 748, CEP 05508-000 São Paulo, SP, Brazil. Electronic address: denistadeu@gmail.com.
4
Departamento de Química Fundamental - Instituto de Química - Universidade de São Paulo, Av. Prof. Lineu Prestes, 748, CEP 05508-000 São Paulo, SP, Brazil. Electronic address: claudemi@iq.usp.br.

Abstract

A capillary electrophoresis-tandem mass spectrometry (CE-MS/MS) method for the simultaneous assessment of nine biogenic amines (spermine, spermidine, putrescine, cadaverine, histamine, phenylethylamine, tryptamine, tyramine, and urocanic acid) in commercial samples of beer and wine is introduced. The samples were submitted to a simple clean-up step with poly(vinylpolypyrrolidone) followed by filtration. Electrophoretic separation in a polyvinyl alcohol (PVA)-coated capillary using 0.5 mol L(-1) acetic acid (pH 2.5) as background electrolyte and detection by electrospray-tandem mass spectrometry was employed. The range of the correlation coefficients of the calibration curves of the analyzed compounds was 0.996-0.999, and the limits of detection and limits of quantification were in the range of 1-2 μg L(-1) and 3-8 μg L(-1), respectively. The recovery values for samples spiked at three concentration levels (0.2, 0.5, and 1.0 mg L(-1)) ranged from 87 to 113% with standard deviation not greater than 5.8%. The use of a PVA-coated silica capillary allows suppressing the electroosmotic flow and, consequently, increasing of the separation efficiency. The method was successfully used to determine biogenic amines in commercial samples of beer and wine.

KEYWORDS:

2-Phenyl-ethylamine (PubChem CID 9075); Beer; Biogenic amines; Cadaverine (PubChem CID 273); Capillary electrophoresis; Histamine (PubChem CID 774); Putrescine (PubChem CID 1045); Spermidine (PubChem CID 1102); Spermine (PubChem CID 1103); Tandem mass spectrometry; Tryptamine (PubChem CID 1150); Tyramine (PubChem CID 5610); Urocanic acid (PubChem CID 736715); Wine

PMID:
26362807
DOI:
10.1016/j.chroma.2015.08.065
[Indexed for MEDLINE]

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