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J Gen Microbiol. 1989 Dec;135(12):3329-36.

Plasmid-mediated chloramphenicol resistance in Staphylococcus hyicus.

Author information

1
Institut für Bakteriologie, Immunologie der Justus-Liebig Universität Giessen, FRG.

Abstract

A small plasmid of 3.95 kb, encoding resistance to chloramphenicol (Cm) was detected in three of 33 Staphylococcus hyicus strains. The plasmid in each of the three strains was indistinguishable by Southern-blot hybridization and restriction enzyme analysis. It was shown by curing and by transformation to specify resistance to Cm. A preliminary restriction map of the plasmid, designated pSC2, is presented. Chloramphenicol acetyltransferase was demonstrated by enzyme assay and by SDS-PAGE of cell-free lysates of pSC2 transformants.

PMID:
2636262
DOI:
10.1099/00221287-135-12-3329
[Indexed for MEDLINE]

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