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Immunity. 2015 Sep 15;43(3):527-40. doi: 10.1016/j.immuni.2015.08.011. Epub 2015 Sep 8.

BALB/c and C57BL/6 Mice Differ in Polyreactive IgA Abundance, which Impacts the Generation of Antigen-Specific IgA and Microbiota Diversity.

Author information

1
Department of Experimental Oncology and Immunotherapy Programme, IEO, Via Adamello 16, 20139 Milan, Italy.
2
Institute of Biomembranes and Bioenergetics, Consiglio Nazionale delle Ricerche, Via Amendola 165/A, 70126 Bari, Italy.
3
Department of Experimental Oncology and Immunotherapy Programme, IEO, Via Adamello 16, 20139 Milan, Italy; Groningen Biomolecular Sciences and Biotechnology, Faculty of Mathematics and Natural Sciences, Nijenborgh 7, 9700 CC Groningen, the Netherlands.
4
Department of Biosciences, Biotechnology and Biopharmaceutics, University of Bari, Via Orabona 4, 70125 Bari, Italy.
5
Institute of Immunology, Hannover Medical School, Carl-Neuberg-Stra. 1, 30625 Hannover, Germany.
6
Institute of Molecular Medicine, RWTH Aachen University, Carl-Neuberg-Str. 1, 52074 Aachen, Germany.
7
Department of Experimental Medicine, University of Perugia, Polo Unico Sant'Andrea delle Fratte, 06132 Perugia, Italy.
8
Institute of Biomembranes and Bioenergetics, Consiglio Nazionale delle Ricerche, Via Amendola 165/A, 70126 Bari, Italy; Department of Biosciences, Biotechnology and Biopharmaceutics, University of Bari, Via Orabona 4, 70125 Bari, Italy.
9
Department of Experimental Oncology and Immunotherapy Programme, IEO, Via Adamello 16, 20139 Milan, Italy; Dipartimento di Scienze della salute, Universita' di Milano, via Rudini 8, 20142 Milan, Italy. Electronic address: maria.rescigno@ieo.eu.

Abstract

The interrelationship between IgAs and microbiota diversity is still unclear. Here we show that BALB/c mice had higher abundance and diversity of IgAs than C57BL/6 mice and that this correlated with increased microbiota diversity. We show that polyreactive IgAs mediated the entrance of non-invasive bacteria to Peyer's patches, independently of CX3CR1(+) phagocytes. This allowed the induction of bacteria-specific IgA and the establishment of a positive feedback loop of IgA production. Cohousing of mice or fecal transplantation had little or no influence on IgA production and had only partial impact on microbiota composition. Germ-free BALB/c, but not C57BL/6, mice already had polyreactive IgAs that influenced microbiota diversity and selection after colonization. Together, these data suggest that genetic predisposition to produce polyreactive IgAs has a strong impact on the generation of antigen-specific IgAs and the selection and maintenance of microbiota diversity.

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PMID:
26362264
DOI:
10.1016/j.immuni.2015.08.011
[Indexed for MEDLINE]
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