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J Theor Biol. 2015 Dec 7;386:18-24. doi: 10.1016/j.jtbi.2015.08.028. Epub 2015 Sep 8.

Homology modeling, functional annotation and comparative genomics of outer membrane protein H of Pasteurella multocida.

Author information

1
Animal Biotechnology Center, Department of Veterinary Physiology and Biochemistry, College of Veterinary and Animal Sciences, G. B. Pant University of Agriculture and Technology, Pantnagar 263145, India; Department of Veterinary Microbiology, College of Veterinary and Animal Sciences, G. B. Pant University of Agriculture and Technology, Pantnagar 263145, India. Electronic address: vetbhaskar@gmail.com.
2
Department of Veterinary Microbiology, College of Veterinary and Animal Sciences, G. B. Pant University of Agriculture and Technology, Pantnagar 263145, India.

Abstract

Pasteurella multocida is an important pathogen of animals and humans. Outer Membrane Protein (Omp) H is a major conserved protein in the envelope of P. multocida and has been commonly targeted as a protective antigen. However, not much is known about its structure and function due to the difficulties that are typically associated with obtaining sufficient amounts of purified prokaryotic transmembrane proteins. The present work is aimed at studying the OmpH using an in silico approach and consolidate the findings in light of existing experimental evidences. Our study describes the first 3D model of the P. multocida OmpH obtained through a combination of several in silico modeling approaches. From our results, OmpH of P. multocida could be classified as a homotrimeric, 16 stranded, β-barrel porin involved in the non-specific transport of small, hydrophilic molecules, serving essential osmoregulatory function. Moreover, very small homologous sequences could be identified in the host proteome, strengthening the probability of a successful OmpH-based vaccine against the pathogen with remote chances of cross-reaction to host proteins.

KEYWORDS:

Annotation; Homology modeling; Outer membrane protein; Pasteurella multocida; Porin

PMID:
26362105
DOI:
10.1016/j.jtbi.2015.08.028
[Indexed for MEDLINE]

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