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Biomaterials. 2015 Dec;72:125-37. doi: 10.1016/j.biomaterials.2015.08.053. Epub 2015 Aug 31.

Adipose-derived stromal cells mediate in vivo adipogenesis, angiogenesis and inflammation in decellularized adipose tissue bioscaffolds.

Author information

1
Department of Chemical Engineering, Queen's University, 19 Division Street, Kingston, Ontario, Canada, K7L 3N6; Human Mobility Research Centre, Kingston General Hospital, 76 Stuart Street, Kingston, Ontario, Canada, K7L 2V7.
2
Department of Chemical Engineering, Queen's University, 19 Division Street, Kingston, Ontario, Canada, K7L 3N6.
3
Department of Chemical Engineering, Queen's University, 19 Division Street, Kingston, Ontario, Canada, K7L 3N6; Human Mobility Research Centre, Kingston General Hospital, 76 Stuart Street, Kingston, Ontario, Canada, K7L 2V7; Department of Chemical and Biochemical Engineering, Thompson Engineering Building, The University of Western Ontario, London, Ontario, Canada, N6A 5B9; Department of Anatomy & Cell Biology, Schulich School of Medicine & Dentistry, The University of Western Ontario, London, Ontario, Canada, N6A 5C1. Electronic address: lauren.flynn@uwo.ca.

Abstract

Decellularized adipose tissue (DAT) has shown promise as an adipogenic bioscaffold for soft tissue augmentation and reconstruction. The objective of the current study was to investigate the effects of allogeneic adipose-derived stem/stromal cells (ASCs) on in vivo fat regeneration in DAT bioscaffolds using an immunocompetent rat model. ASC seeding significantly enhanced angiogenesis and adipogenesis, with cell tracking studies indicating that the newly-forming tissues were host-derived. Incorporating ASCs also mediated the inflammatory response and promoted a more constructive macrophage phenotype. A fraction of the CD163(+) macrophages in the implants expressed adipogenic markers, with higher levels of this "adipocyte-like" phenotype in proximity to the developing adipose tissues. Our results indicate that the combination of ASCs and adipose extracellular matrix (ECM) provides an inductive microenvironment for adipose regeneration mediated by infiltrating host cell populations. The DAT scaffolds are a useful tissue-specific model system for investigating the mechanisms of in vivo adipogenesis that may help to develop a better understanding of this complex process in the context of both regeneration and disease. Overall, combining adipose-derived matrices with ASCs is a highly promising approach for the in situ regeneration of host-derived adipose tissue.

KEYWORDS:

Adipose tissue engineering; ECM (extracellular matrix); Macrophage; Mesenchymal stem cell; Scaffold

[Indexed for MEDLINE]

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