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Bioinformatics. 2015 Dec 15;31(24):4014-6. doi: 10.1093/bioinformatics/btv537. Epub 2015 Sep 10.

Cas-Designer: a web-based tool for choice of CRISPR-Cas9 target sites.

Author information

1
Department of Chemistry.
2
Department of Chemistry, Institute for Materials Design, Hanyang University, Seoul, South Korea.
3
Center for Genome Engineering, Institute for Basic Science, Seoul, South Korea and Department of Chemistry, Seoul National University, Seoul, South Korea.

Abstract

We present Cas-Designer, a user-friendly program to aid researchers in choosing appropriate target sites in a gene of interest for type II CRISPR/Cas-derived RNA-guided endonucleases, which are now widely used for biomedical research and biotechnology. Cas-Designer rapidly provides the list of all possible guide RNA sequences in a given input DNA sequence and their potential off-target sites including bulge-type sites in a genome of choice. In addition, the program assigns an out-of-frame score to each target site to help users choose appropriate sites for gene knockout. Cas-Designer shows the results in an interactive table and provides user-friendly filter functions.

AVAILABILITY AND IMPLEMENTATION:

Free access at http://rgenome.net/cas-designer/.

PMID:
26358729
DOI:
10.1093/bioinformatics/btv537
[Indexed for MEDLINE]

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