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FEBS Lett. 2015 Oct 7;589(20 Pt B):3126-32. doi: 10.1016/j.febslet.2015.08.039. Epub 2015 Sep 7.

COX assembly factor ccdc56 regulates mitochondrial morphology by affecting mitochondrial recruitment of Drp1.

Author information

1
Department of Protein Biochemistry, Institute of Life Science, Kurume University, Kurume 839-0864, Japan.
2
Department of Chemistry, Faculty of Sciences, Kyushu University, Fukuoka 812-8581, Japan.
3
INSERM, Unité 873, Grenoble F-38054, France.
4
Department of Protein Biochemistry, Institute of Life Science, Kurume University, Kurume 839-0864, Japan. Electronic address: ishihara_naotada@kurume-u.ac.jp.
5
Department of Chemistry, Faculty of Sciences, Kyushu University, Fukuoka 812-8581, Japan. Electronic address: kuge@chem.kyushu-univ.jp.

Abstract

Mitochondria are dynamic organelles that alter their morphology in response to cellular signaling and differentiation through balanced fusion and fission. In this study, we found that the mitochondrial inner membrane ATPase ATAD3A interacted with ccdc56/MITRAC12/COA3, a subunit of the cytochrome oxidase (COX)-assembly complex. Overproduction of ccdc56 in HeLa cells resulted in fragmented mitochondrial morphology, while mitochondria were highly elongated in ccdc56-repressed cells by the defective recruitment of the fission factor Drp1. We also found that mild and chronic inhibition of COX led to mitochondrial elongation, as seen in ccdc56-repressed cells. These results indicate that ccdc56 positively regulates mitochondrial fission via regulation of COX activity and the mitochondrial recruitment of Drp1, and thus, suggest a novel relationship between COX assembly and mitochondrial morphology.

KEYWORDS:

Cytochrome oxidase (COX); Drpl; Mitochondria; Mitochondrial fission; Organelle

PMID:
26358295
DOI:
10.1016/j.febslet.2015.08.039
[Indexed for MEDLINE]
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