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Proc Biol Sci. 2015 Sep 22;282(1815). pii: 20150746. doi: 10.1098/rspb.2015.0746.

Ectodysplasin signalling genes and phenotypic evolution in sculpins (Cottus).

Author information

1
Department for Evolutionary Genetics, Max Planck Institute for Evolutionary Biology, August-Thienemann Strasse 2, 24306 Plön, Germany Ministry of Education Key Laboratory of Marine Genetics and Breeding, College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China.
2
Center for Integrative Bioinformatics Vienna, Max F. Perutz Laboratories, University of Vienna, Medical University of Vienna, 1030 Vienna, Austria Cold Spring Harbor Laboratory, Simons Center for Quantitative Biology, Cold Spring Harbor, New York, NY, USA.
3
Cologne Center for Genomics, University of Cologne, 50931 Cologne, Germany Institute of Human Genetics, University of Cologne, 50931 Cologne, Germany.
4
Department for Evolutionary Genetics, Max Planck Institute for Evolutionary Biology, August-Thienemann Strasse 2, 24306 Plön, Germany nolte@evolbio.mpg.de.

Abstract

Despite their deeply conserved function among vertebrates, ectodysplasin (Eda) signalling genes are involved in microevolutionary change in humans and sticklebacks. If such a dual role is common, Eda signalling genes constitute hotspots for morphological evolution. Variation in sculpin (Cottus) skin prickling and body shape resembles patterns caused by variation in Eda signalling in sticklebacks. We mapped Eda signalling genes and performed quantitative trait locus mapping in crosses between Cottus rhenanus and Cottus perifretum. A genomic region containing the Eda receptor (Edar) was strongly associated with prickling and contributed to shape. The expression of Edar in developing prickles and skeletal elements in Cottus was confirmed by in situ hybridization. Coding sequence changes between Edar alleles in C. rhenanus and C. perifretum exceeded sequence differentiation in other vertebrates. However, it is likely that additional genetic elements besides coding changes affect the phenotypic variation. Although the phenotype in a natural hybrid lineage between C. rhenanus and C. perifretum resembles C. perifretum, the respective coding Edar alleles are not fully fixed (88.6%). Hence, our results support an involvement of Eda signalling in microevolutionary changes, but imply that the Edar gene is affected by multiple evolutionary processes that vary among freshwater sculpins.

KEYWORDS:

body shape; convergent evolution; hybrid intermediacy; lateral line; scales

PMID:
26354934
PMCID:
PMC4614746
DOI:
10.1098/rspb.2015.0746
[Indexed for MEDLINE]
Free PMC Article

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