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Plant Physiol. 2015 Nov;169(3):1524-31. doi: 10.1104/pp.15.01081. Epub 2015 Sep 9.

Discovering New Biology through Sequencing of RNA.

Author information

  • 1Institute of Plant Biochemistry, Cluster of Excellence on Plant Science, Heinrich-Heine-Universit√§t, D-40231 Duesseldorf, Germany andreas.weber@hhu.de.

Abstract

Sequencing of RNA (RNA-Seq) was invented approximately 1 decade ago and has since revolutionized biological research. This update provides a brief historic perspective on the development of RNA-Seq and then focuses on the application of RNA-Seq in qualitative and quantitative analyses of transcriptomes. Particular emphasis is given to aspects of data analysis. Since the wet-lab and data analysis aspects of RNA-Seq are still rapidly evolving and novel applications are continuously reported, a printed review will be rapidly outdated and can only serve to provide some examples and general guidelines for planning and conducting RNA-Seq studies. Hence, selected references to frequently update online resources are given.

PMID:
26353759
PMCID:
PMC4634082
DOI:
10.1104/pp.15.01081
[PubMed - indexed for MEDLINE]
Free PMC Article
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