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ACS Chem Biol. 2015 Nov 20;10(11):2468-79. doi: 10.1021/acschembio.5b00525. Epub 2015 Sep 8.

Biosynthesis of the Novel Macrolide Antibiotic Anthracimycin.

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1
Department of Molecular Microbiology, John Innes Centre , Norwich Research Park, Norwich, Norfolk NR4 7UH, United Kingdom.

Abstract

We report the identification of the biosynthetic gene cluster for the unusual antibiotic anthracimycin (atc) from the marine derived producer strain Streptomyces sp. T676 isolated off St. John's Island, Singapore. The 53 253 bps atc locus includes a trans-acyltransferase (trans-AT) polyketide synthase (PKS), and heterologous expression in Streptomyces coelicolor resulted in anthracimycin production. Analysis of the atc cluster revealed that anthracimycin is likely generated by four PKS gene products AtcC-AtcF without involvement of post-PKS tailoring enzymes, and a biosynthetic pathway is proposed. The availability of the atc cluster provides a basis for investigating the biosynthesis of anthracimycin and its subsequent bioengineering to provide novel analogues with improved pharmacological properties.

PMID:
26349074
DOI:
10.1021/acschembio.5b00525
[Indexed for MEDLINE]
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