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Front Microbiol. 2015 Aug 18;6:766. doi: 10.3389/fmicb.2015.00766. eCollection 2015.

MinION nanopore sequencing of an influenza genome.

Author information

1
Institute of Environmental Science and Research, National Centre for Biosecurity and Infectious Disease , Upper Hutt, New Zealand.
2
WHO Collaborating Centre for Reference and Research on Influenza, Victorian Infectious Diseases Reference Laboratory, Peter Doherty Institute for Infection and Immunity , Melbourne, VIC, Australia.

Abstract

Influenza epidemics and pandemics have significant impacts on economies, morbidity and mortality worldwide. The ability to rapidly and accurately sequence influenza viruses is instrumental in the prevention and mitigation of influenza. All eight influenza genes from an influenza A virus were amplified by PCR simultaneously and then subjected to sequencing on a MinION nanopore sequencer. A complete influenza virus genome was obtained that shared greater than 99% identity with sequence data obtained from Illumina MiSeq and traditional Sanger-sequencing. The laboratory infrastructure and computing resources used to perform this experiment on the MinION nanopore sequencer would be available in most molecular laboratories around the world. Using this system, the concept of portability, and thus sequencing influenza viruses in the clinic or field is now tenable.

KEYWORDS:

MinION; influenza; nanopore sequencing; virus; whole genome sequencing

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