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Immunobiology. 2016 Jan;221(1):12-22. doi: 10.1016/j.imbio.2015.08.007. Epub 2015 Aug 24.

Characterization of a novel canine T-cell line established from a spontaneously occurring aggressive T-cell lymphoma with large granular cell morphology.

Author information

1
UPSP ICE 2011-03-101, Oncologie, VetAgro Sup, Campus Vétérinaire, 1 Avenue Bourgelat 69280 Marcy l'Etoile, France. Electronic address: catherine.bonnefont@vetagro-sup.fr.
2
UPSP ICE 2011-03-101, Oncologie, VetAgro Sup, Campus Vétérinaire, 1 Avenue Bourgelat 69280 Marcy l'Etoile, France.
3
Dendritics, 60 Avenue Rockefeller, 69008 Lyon, France.
4
INRA, UMR754 "Rétrovirus et Pathologie Comparée", Université Lyon 1, 50 Avenue Tony Garnier, 69007 Lyon, France.

Abstract

Dogs with lymphoma are established as good model for human non-Hodgkin lymphoma studies. Canine cell lines derived from lymphomas may be valuable tools for testing new therapeutic drugs. In this context, we established a canine T-cell line, PER-VAS, from a primary aggressive T-cell lymphoma with large granular morphology. Flow cytometric analysis revealed a stable immunophenotype: PER-VAS cells were positively labelled for CD5, CD45, MHC II and TLR3, and were negative for CD3, CD4 and CD8 expression. Although unstable along the culture process, IL-17 and MMP12 proteins were detectable as late as at passages 280 and 325i.e. respectively 24 and 29 months post isolation. At passage 325, PER-VAS cells maintained the expression of IL-17, CD3, CD56, IFNγ and TNFα mRNAs as shown by RT-PCR analysis. Stable rearrangement of the TCRγ gene has been evidenced by PCR. PER-VAS cells have a high proliferation index with a doubling time of 16.5h and were tumorigenic in Nude mice. Compared to the canine cell lines already reported, PER-VAS cells display an original expression pattern, close to NKT cells, which makes them valuable tools for in vitro comparative research on lymphomas.

KEYWORDS:

CD56 mRNA; Cell line; Dog; IL-17; T-cell lymphoma

PMID:
26345430
DOI:
10.1016/j.imbio.2015.08.007
[Indexed for MEDLINE]
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