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J Cell Sci. 2015 Oct 15;128(20):3707-13. doi: 10.1242/jcs.171314. Epub 2015 Sep 7.

A new phosphate-starvation response in fission yeast requires the endocytic function of myosin I.

Author information

1
Nuclear Dynamics, Babraham Institute, Cambridge CB22 3AT, USA.
2
Imaging Facility, Babraham Institute, Cambridge CB22 3AT, USA.
3
Nuclear Dynamics, Babraham Institute, Cambridge CB22 3AT, USA patrick.varga-weisz@babraham.ac.uk.

Abstract

Endocytosis is essential for uptake of many substances into the cell, but how it links to nutritional signalling is poorly understood. Here, we show a new role for endocytosis in regulating the response to low phosphate in Schizosaccharomyces pombe. Loss of function of myosin I (Myo1), Sla2/End4 or Arp2, proteins involved in the early steps of endocytosis, led to increased proliferation in low-phosphate medium compared to controls. We show that once cells are deprived of phosphate they undergo a quiescence response that is dependent on the endocytic function of Myo1. Transcriptomic analysis revealed a wide perturbation of gene expression with induction of stress-regulated genes upon phosphate starvation in wild-type but not Δmyo1 cells. Thus, endocytosis plays a pivotal role in mediating the cellular response to nutrients, bridging the external environment and internal molecular functions of the cell.

KEYWORDS:

Endocytosis; Myosin; Phosphate sensing

PMID:
26345368
PMCID:
PMC4631780
DOI:
10.1242/jcs.171314
[Indexed for MEDLINE]
Free PMC Article

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