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Neurosci Bull. 2015 Dec;31(6):685-96. doi: 10.1007/s12264-015-1558-0. Epub 2015 Sep 7.

Increasing the effectiveness of intracerebral injections in adult and neonatal mice: a neurosurgical point of view.

Mathon B1,2,3,4,5, Nassar M6,7,8,9, Simonnet J6,7,8,9, Le Duigou C6,7,8,9, Clemenceau S10, Miles R6,7,8,9, Fricker D6,7,8,9.

Author information

1
Department of Neurosurgery, Groupe Hospitalier, Universitaire de La Pitié-Salpêtrière, APHP, F-75013, Paris, France. bertrand.mathon@neurochirurgie.fr.
2
Inserm, U 1127, F-75013, Paris, France. bertrand.mathon@neurochirurgie.fr.
3
CNRS, UMR 7225, F-75013, Paris, France. bertrand.mathon@neurochirurgie.fr.
4
Sorbonne Universités, UPMC Univ Paris 06, UMR S 1127, F-75013, Paris, France. bertrand.mathon@neurochirurgie.fr.
5
Institut du Cerveau et de la Moelle épinière, ICM, F-75013, Paris, France. bertrand.mathon@neurochirurgie.fr.
6
Inserm, U 1127, F-75013, Paris, France.
7
CNRS, UMR 7225, F-75013, Paris, France.
8
Sorbonne Universités, UPMC Univ Paris 06, UMR S 1127, F-75013, Paris, France.
9
Institut du Cerveau et de la Moelle épinière, ICM, F-75013, Paris, France.
10
Department of Neurosurgery, Groupe Hospitalier, Universitaire de La Pitié-Salpêtrière, APHP, F-75013, Paris, France.

Abstract

Intracerebral injections of tracers or viral constructs in rodents are now commonly used in the neurosciences and must be executed perfectly. The purpose of this article is to update existing protocols for intracerebral injections in adult and neonatal mice. Our procedure for stereotaxic injections in adult mice allows the investigator to improve the effectiveness and safety, and save time. Furthermore, for the first time, we describe a two-handed procedure for intracerebral injections in neonatal mice that can be performed by a single operator in a very short time. Our technique using the stereotaxic arm allows a higher precision than freehand techniques previously described. Stereotaxic injections in adult mice can be performed in 20 min and have >90% efficacy in targeting the injection site. Injections in neonatal mice can be performed in 5 min. Efficacy depends on the difficulty of precisely localizing the injection sites, due to the small size of the animal. We describe an innovative, effortless, and reproducible surgical protocol for intracerebral injections in adult and neonatal mice.

KEYWORDS:

neonatal brain; neurosciences; newborn mice; rodents; stereotaxic injection; surgery

PMID:
26345180
PMCID:
PMC5563725
DOI:
10.1007/s12264-015-1558-0
[Indexed for MEDLINE]
Free PMC Article

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