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Int J Environ Res Public Health. 2015 Aug 28;12(9):10575-86. doi: 10.3390/ijerph120910575.

Trichloroethylene and Its Oxidative Metabolites Enhance the Activated State and Th1 Cytokine Gene Expression in Jurkat Cells.

Pan Y1,2, Wei X3,4, Hao W5,6.

Author information

1
Department of Toxicology, School of Public Health, Peking University, Beijing 100191, China. yaopan408@gmail.com.
2
Beijing Key Laboratory of Toxicological Research and Risk Assessment for Food Safety, Beijing 100191, China. yaopan408@gmail.com.
3
Department of Toxicology, School of Public Health, Peking University, Beijing 100191, China. weixt@263.net.
4
Beijing Key Laboratory of Toxicological Research and Risk Assessment for Food Safety, Beijing 100191, China. weixt@263.net.
5
Department of Toxicology, School of Public Health, Peking University, Beijing 100191, China. whao@bjmu.edu.cn.
6
Beijing Key Laboratory of Toxicological Research and Risk Assessment for Food Safety, Beijing 100191, China. whao@bjmu.edu.cn.

Abstract

Trichloroethylene (TCE) is an occupational and ubiquitous environmental contaminant, and TCE exposure will increase the risk of autoimmune diseases and allergic diseases. T cells play an important role in the pathogenesis of TCE-related immune disorders, but the effect of TCE and its oxidative metabolites, trichloroacetic acid (TCA) and dichloroacetic acid (DCA), on the activation of human T cells is still unknown. In this study, Jurkat cells were pre-treated with TCE, TCA and DCA overnight and then stimulated with phorbol 12-myristate 13-acetate and ionomycin for another 4, 8 and 24 hours. IL-2 secretion was detected by ELISA; the expressions of CD25 and CD69 were tested by flow cytometry; and IFN-γ and IL-2 mRNA expression levels were investigated by real-time PCR. The results showed that TCE and its oxidative metabolites, TCA and DCA, significantly enhanced IL-2 releasing and the expression of T cell activation markers, CD25 and CD69. Consistent with this result, these compounds markedly up-regulated the expression levels of IFN-γ and IL-2 mRNA. Collectively, these findings suggest that TCE and its metabolites, TCA and DCA, might enhance the activation of T cells and disrupt various activities of peripheral T cells.

KEYWORDS:

IL-2; Jurkat cells; dichloroacetic acid; trichloroacetic acid; trichloroethylene

PMID:
26343699
PMCID:
PMC4586629
DOI:
10.3390/ijerph120910575
[Indexed for MEDLINE]
Free PMC Article

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