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Stem Cell Res. 2015 Sep;15(2):403-18. doi: 10.1016/j.scr.2015.08.004. Epub 2015 Aug 15.

Characterization of DLK1(PREF1)+/CD34+ cells in vascular stroma of human white adipose tissue.

Author information

1
Division for Clinical and Functional Anatomy, Department for Anatomy, Histology and Embryology, Medical University of Innsbruck, Austria.
2
Cell Metabolism and Differentiation Research Group, Institute for Biomedical Aging Research, University of Innsbruck, Austria.
3
Department of Plastic, Reconstructive and Aesthetic Surgery, Medical University of Innsbruck, Austria.
4
Department of Internal Medicine V, Medical University of Innsbruck, Austria.
5
Cell Metabolism and Differentiation Research Group, Institute for Biomedical Aging Research, University of Innsbruck, Austria. Electronic address: werner.zwerschke@uibk.ac.at.

Abstract

Sorting of native (unpermeabilized) SVF-cells from human subcutaneous (s)WAT for cell surface staining (cs) of DLK1 and CD34 identified three main populations: ~10% stained cs-DLK1+/cs-CD34-, ~20% cs-DLK1+/cs-CD34+dim and ~45% cs-DLK1-/cs-CD34+. FACS analysis after permeabilization showed that all these cells stained positive for intracellular DLK1, while CD34 was undetectable in cs-DLK1+/cs-CD34- cells. Permeabilized cs-DLK1-/cs-CD34+ cells were positive for the pericyte marker α-SMA and the mesenchymal markers CD90 and CD105, albeit CD105 staining was dim (cs-DLK1-/cs-CD34+/CD90+/CD105+dim/α-SMA+/CD45-/CD31-). Only these cells showed proliferative and adipogenic capacity. Cs-DLK1+/cs-CD34- and cs-DLK1+/cs-CD34+dim cells were also α-SMA+ but expressed CD31, had a mixed hematopoietic and mesenchymal phenotype, and could neither proliferate nor differentiate into adipocytes. Histological analysis of sWAT detected DLK1+/CD34+ and DLK1+/CD90+ cells mainly in the outer ring of vessel-associated stroma and at capillaries. DLK1+/α-SMA+ cells were localized in the CD34- perivascular ring and in adventitial vascular stroma. All these DLK1+ cells possess a spindle-shaped morphology with extremely long processes. DLK1+/CD34+ cells were also detected in vessel endothelium. Additionally, we show that sWAT contains significantly more DLK1+ cells than visceral (v)WAT. We conclude that sWAT has more DKL1+ cells than vWAT and contains different DLK1/CD34 populations, and only cs-DLK1-/cs-CD34+/CD90+/CD105+dim/α-SMA+/CD45-/CD31- cells in the adventitial vascular stroma exhibit proliferative and adipogenic capacity.

KEYWORDS:

Adipogenesis; Adipose-derived stromal/progenitor cells; CD34; CD90; Clonal expansion; DLK1; Vascular niche; α-SMA

PMID:
26342195
DOI:
10.1016/j.scr.2015.08.004
[Indexed for MEDLINE]
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