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Biol Open. 2015 Sep 4;4(10):1222-8. doi: 10.1242/bio.012096.

The rostral migratory stream generates hippocampal CA1 pyramidal-like neurons in a novel organotypic slice co-culture model.

Author information

1
Institute of Anatomy and Cell Biology, Albert-Ludwigs-University Freiburg, D-79104 Freiburg, Germany Department of Neuropathology, Albert-Ludwigs-University Freiburg, D-79106 Freiburg, Germany ilyassingec@gmail.com.
2
Department of Neuropathology, Albert-Ludwigs-University Freiburg, D-79106 Freiburg, Germany.
3
Institute for Integrative Neuroanatomy, Charité, D-10117 Berlin, Germany.
4
Center for Molecular Neurobiology (ZMNH), University Medical Center Hamburg-Eppendorf, D-20251 Hamburg, Germany.

Abstract

The mouse subventricular zone (SVZ) generates large numbers of neuroblasts, which migrate in a distinct pathway, the rostral migratory stream (RMS), and replace specific interneurons in the olfactory bulb (OB). Here, we introduce an organotypic slice culture model that directly connects the RMS to the hippocampus as a new destination. RMS neuroblasts widely populate the hippocampus and undergo cellular differentiation. We demonstrate that RMS cells give rise to various neuronal subtypes and, surprisingly, to CA1 pyramidal neurons. Pyramidal neurons are typically generated before birth and are lost in various neurological disorders. Hence, this unique slice culture model enables us to investigate their postnatal genesis under defined in vitro conditions from the RMS, an unanticipated source for hippocampal pyramidal neurons.

KEYWORDS:

Cellular plasticity; Microenvironment; Neuronal differentiation; Organotypic slice culture; Pyramidal neuron; Rostral migratory stream

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