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Int J Clin Exp Pathol. 2015 Jul 1;8(7):8663-70. eCollection 2015.

Podoplanin increases migration and angiogenesis in malignant glioma.

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Department of Neurosurgery, University of Cologne Germany.
Department of Gynaecology, University Medical Center Hamburg-Eppendorf, Germany.
Department of Neurosurgery, Ludwig-Maximilian-University Munich Germany.
Helmholtz Zentrum Munich Germany.
Department of Internal Medicine, Ludwig-Maximilian-University Munich Germany.
Department of Pediatrics, Technical University Munich Germany.


Expression of podoplanin in glial brain tumors is grade dependent. While serving as a marker for tumor progression and modulating invasion in various neoplasms, little is known about podoplanin function in gliomas. Therefore we stably transfected two human glioma cell lines (U373MG and U87MG) with expression plasmids encoding podoplanin. The efficacy of transfection was confirmed by FACS analysis, PCR and immunocytochemistry. Cells were then sorted for highly podoplanin expressing cells (U373P(high)/U87P(high)). Transfection did not influence the production of pro-angiogenic factors including VEGF, VEGF-C and D. Also, expression of VEGF receptors (VEGFR) remained unchanged except for U87P(high), where a VEGFR3 expression was induced. U373P(high) showed significantly reduced proliferation as compared to mock transfected group. By contrast, podoplanin significantly increased migration and invasion into collagen matrix. Furthermore, conditioned media from P(high) glioma cells strongly induced tube formation on matrigel. In conclusion, podoplanin increased migration of tumor cells and enhanced tube formation activity in endothelial cells independent from VEGF. Thus, podoplanin expression may be an important step in tumor progression.


Podoplanin; angiogenesis; glioma

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