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Mol Biol Cell. 2015 Nov 1;26(21):3777-87. doi: 10.1091/mbc.E15-02-0115. Epub 2015 Sep 2.

Global analysis of asymmetric RNA enrichment in oocytes reveals low conservation between closely related Xenopus species.

Author information

1
Institute of Developmental Biochemistry, University Medical Center Göttingen, 37077 Göttingen, Germany mclauss1@gwdg.de tpieler@gwdg.de.
2
Microarray and Deep-Sequencing Core Facility, University Medical Center Göttingen, 37077 Göttingen, Germany.

Abstract

RNAs that localize to the vegetal cortex during Xenopus laevis oogenesis have been reported to function in germ layer patterning, axis determination, and development of the primordial germ cells. Here we report on the genome-wide, comparative analysis of differentially localizing RNAs in Xenopus laevis and Xenopus tropicalis oocytes, revealing a surprisingly weak degree of conservation in respect to the identity of animally as well as vegetally enriched transcripts in these closely related species. Heterologous RNA injections and protein binding studies indicate that the different RNA localization patterns in these two species are due to gain/loss of cis-acting localization signals rather than to differences in the RNA-localizing machinery.

PMID:
26337391
PMCID:
PMC4626063
DOI:
10.1091/mbc.E15-02-0115
[Indexed for MEDLINE]
Free PMC Article

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