Format

Send to

Choose Destination
See comment in PubMed Commons below
J Genet Genomics. 2015 Aug 20;42(8):413-21. doi: 10.1016/j.jgg.2015.06.005. Epub 2015 Jun 26.

The application of CRISPR-Cas9 genome editing in Caenorhabditis elegans.

Author information

  • 1Division of Biological Sciences, Section of Cell and Developmental Biology, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA. Electronic address: suhongxu@gmail.com.

Abstract

Genome editing using the Cas9 endonuclease of Streptococcus pyogenes has demonstrated unparalleled efficacy and facility for modifying genomes in a wide variety of organisms. Caenorhabditis elegans is one of the most convenient multicellular organisms for genetic analysis, and the application of this novel genome editing technique to this organism promises to revolutionize analysis of gene function in the future. CRISPR-Cas9 has been successfully used to generate imprecise insertions and deletions via non-homologous end-joining mechanisms and to create precise mutations by homology-directed repair from donor templates. Key variables are the methods used to deliver the Cas9 endonuclease and the efficiency of the single guide RNAs. CRISPR-Cas9-mediated editing appears to be highly specific in C. elegans, with no reported off-target effects. In this review, I briefly summarize recent progress in CRISPR-Cas9-based genome editing in C. elegans, highlighting technical improvements in mutagenesis and mutation detection, and discuss potential future applications of this technique.

KEYWORDS:

C. elegans; CRISPR; Cas9; Genome editing; Homology-directed repair (HDR); Non-homologous end-joining (NHEJ); Somatic mutation

PMID:
26336798
PMCID:
PMC4560834
DOI:
10.1016/j.jgg.2015.06.005
[PubMed - indexed for MEDLINE]
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Elsevier Science Icon for PubMed Central
    Loading ...
    Support Center