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Microbiome. 2015 Sep 3;3:37. doi: 10.1186/s40168-015-0102-9.

The effect of dietary resistant starch type 2 on the microbiota and markers of gut inflammation in rural Malawi children.

Author information

1
Department of Pediatrics, Washington University, St. Louis, MO, 63110, USA.
2
Department of Pediatrics, Baylor College of Medicine, Houston, TX, 77030, USA.
3
The Genome Institute, Washington University, St. Louis, MO, 63110, USA.
4
NIH/NIGMS Biomedical Mass Spectrometry, Washington University, St. Louis, MO, 63110, USA.
5
Flinders Centre for Innovation in Cancer, Adelaide, Australia.
6
Department of Community Health, College of Medicine, Blantyre, Malawi.
7
Department of Medicine, Washington University, St. Louis, MO, 63110, USA.
8
Department of Pediatrics, Washington University, St. Louis, MO, 63110, USA. manary@kids.wustl.edu.
9
Department of Pediatrics, Baylor College of Medicine, Houston, TX, 77030, USA. manary@kids.wustl.edu.
10
Department of Community Health, College of Medicine, Blantyre, Malawi. manary@kids.wustl.edu.
11
Washington University, School of Medicine, St. Louis, MO, 63110, USA. manary@kids.wustl.edu.

Abstract

BACKGROUND:

Resistant starch (RS) decreases intestinal inflammation in some settings. We tested the hypothesis that gut inflammation will be reduced with dietary supplementation with RS in rural Malawian children. Eighteen stunted 3-5-year-old children were supplemented with 8.5 g/day of RS type 2 for 4 weeks. The fecal samples were analyzed for the microbiota, the microbiome, short chain fatty acids, metabolome, and proteins indicative of inflammation before and after the intervention. Subjects served as their own controls.

RESULTS:

The consumption of RS changed the composition of the microbiota; at the phylum level Actinobacteria increased, while Firmicutes decreased. Among the most prevalent genera, Lactobacillus was increased and Roseburia, Blautia, and Lachnospiracea incertae sedis were decreased. The Shannon H index at the genus level decreased from 2.02 on the habitual diet and 1.76 after the introduction of RS (P < 0.01). Fecal acetate concentration decreased, and fecal propionate concentration increased after RS administration (-5.2 and 2.0 μmol/g, respectively). Fecal calprotectin increased from 29 ± 69 to 89 ± 49 μg/g (P = 0.003) after RS was given. The lipopolysaccharide biosynthesis pathway was upregulated.

CONCLUSIONS:

Our findings do not support the hypothesis that RS reduces gut inflammation in rural Malawian children.

TRIAL REGISTRATION:

ClinicalTrials.gov NCT01811836.

PMID:
26334878
PMCID:
PMC4558878
DOI:
10.1186/s40168-015-0102-9
[Indexed for MEDLINE]
Free PMC Article

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