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Oncotarget. 2015 Oct 20;6(32):32634-45. doi: 10.18632/oncotarget.4728.

Normal mammary epithelial cells promote carcinoma basement membrane invasion by inducing microtubule-rich protrusions.

Lee MH1,2, Wu PH1,2, Gilkes D1,2, Aifuwa I1,2, Wirtz D1,2,3,4.

Author information

1
Johns Hopkins Physical Sciences-Oncology Center, The Johns Hopkins University, Baltimore, Maryland 21218, USA.
2
Department of Chemical and Biomolecular Engineering, The Johns Hopkins University, Baltimore, Maryland 21218, USA.
3
Departments of Pathology and Oncology, The Johns Hopkins School of Medicine, Baltimore, Maryland 21205, USA.
4
Sidney Kimmel Comprehensive Cancer Center, The Johns Hopkins School of Medicine, Baltimore, Maryland 21205, USA.

Abstract

Recent work suggests that the dissemination of tumor cells may occur in parallel with, and even preceed, tumor growth. The mechanism for this early invasion is largely unknown. Here, we find that mammary epithelial cells (MECs) induce neighboring breast carcinoma cells (BCCs) to cross the basement membrane by secreting soluble laminin. Laminin continuously produced by MECs induce long membrane cellular protrusions in BCCs that promote their contractility and invasion into the surrounding matrix. These protrusions depend on microtubule bundles assembled de novo through laminin-integrin β1 signaling. These results describe how non-cancerous MECs can actively participate in the invasive process of BCCs.

KEYWORDS:

cancer invasion; cell-cell interaction; laminin; microtubule; protrusion

PMID:
26334095
PMCID:
PMC4741718
DOI:
10.18632/oncotarget.4728
[Indexed for MEDLINE]
Free PMC Article

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