We have determined hepatitis B virus markers to 107 nursing personnel (90 women and 17 men) of a general hospital. The prevalence was 15.88% (17 persons). Two persons (1.86%) presented HBs-Ag. The prevalence was more low what the prevalences found by other authors of our country. The difference between the two sexes not was significative. The highest prevalences were found in the personnel of outpatient clinics and in the personnel in contact with blood and its derivates. The prevalence more low was found en the nursing personal rooms. The collective of midwives presented the prevalence more height: 37.5%. The prevalence increased generally with age and years in occupation. We associated the low indexes of prevalence found for us with the high percentages of young personnel (39.25%) are more low of 30 years age) and with short years occupation in the hospital (19.62% are more low of 4 years).