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ACS Synth Biol. 2016 Jan 15;5(1):8-14. doi: 10.1021/acssynbio.5b00094. Epub 2015 Sep 15.

A Microfluidic Platform for Long-Term Monitoring of Algae in a Dynamic Environment.

Author information

1
Department of Bioengineering, University of California, San Diego , La Jolla, California 92093, United States.
2
Division of Biological Sciences, University of California, San Diego , La Jolla, California 92093, United States.
3
Center for Circadian Biology, University of California, San Diego , La Jolla, California 92093, United States.
4
BioCircuits Institute, University of California, San Diego , La Jolla, California 92093, United States.

Abstract

Culturing cells in microfluidic "lab-on-a-chip" devices for time lapse microscopy has become a valuable tool for studying the dynamics of biological systems. Although microfluidic technology has been applied to culturing and monitoring a diverse range of bacterial and eukaryotic species, cyanobacteria and eukaryotic microalgae present several challenges that have made them difficult to culture in a microfluidic setting. Here, we present a customizable device for the long-term culturing and imaging of three well characterized strains of cyanobacteria and microalgae. This platform has several advantages over agarose pads and demonstrates great potential for obtaining high quality, single-cell gene expression data of cyanobacteria and algae in precisely controlled, dynamic environments over long time periods.

KEYWORDS:

Chlorella; Synechococcus; Synechocystis; cyanobacteria; dynamics; microfluidics

PMID:
26332284
PMCID:
PMC5249263
DOI:
10.1021/acssynbio.5b00094
[Indexed for MEDLINE]
Free PMC Article

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