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PLoS Negl Trop Dis. 2015 Sep 2;9(9):e0004061. doi: 10.1371/journal.pntd.0004061. eCollection 2015.

A Polymorphism in the Chitotriosidase Gene Associated with Risk of Mycetoma Due to Madurella mycetomatis Mycetoma--A Retrospective Study.

Author information

1
Dept. of Medical Microbiology & Infectious Diseases, Erasmus MC, University Medical Centre Rotterdam, CE Rotterdam, The Netherlands.
2
Mycetoma Research Centre, Khartoum, Sudan.

Abstract

BACKGROUND:

Madurella mycetomatis is the most prevalent causative agent of eumycetoma in Sudan, an infection characterized by the formation of grains. Many patients are exposed to the causative agent, however only a small number develop infection. M. mycetomatis contains chitin in its cell wall, which can trigger the human immune system. Polymorphisms in the genes encoding for the chitin-degrading enzymes chitotriosidase and AMCase were described, resulting in altered chitinase activity. We investigated the association between 4 of these polymorphisms and the incidence of M. mycetomatis mycetoma in a Sudanese population.

METHODOLOGY:

Polymorphisms studied in 112 eumycetoma patients and 103 matched controls included a 24-bp insertion in the chitotriosidase gene (rs3831317), resulting in impaired chitinase activity and single nucleotide polymorphism (SNP) in the AMCase gene (rs61756687), resulting in decreased AMCase activity. Also, a SNP (rs41282492) and a 10-bp insertion in the 5'UTR region of the AMCase gene (rs143789088) were studied, both resulting in increased AMCase activity. DNA was isolated from blood and genotypes were determined using PCR-RFLP.

PRINCIPAL FINDINGS:

Histological staining proved the presence of chitin in the fungal grain. The polymorphism resulting in decreased chitotriosidase activity was associated with increased odds of eumycetoma (odds ratio 2.9; p = 0.004). No association was found for the polymorphisms in the genes for AMCase (all p>0.05).

CONCLUSION:

Decreased chitotriosidase activity was associated with increased risk of M. mycetomatis mycetoma.

PMID:
26332238
PMCID:
PMC4558086
DOI:
10.1371/journal.pntd.0004061
[Indexed for MEDLINE]
Free PMC Article

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