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Curr Protoc Mouse Biol. 2015 Sep 1;5(3):247-270. doi: 10.1002/9780470942390.mo140190.

Diabetic Retinopathy: Retina-Specific Methods for Maintenance of Diabetic Rodents and Evaluation of Vascular Histopathology and Molecular Abnormalities.

Veenstra A1,2,3, Liu H1,3, Lee CA1, Du Y1, Tang J1,2, Kern TS1,2.

Author information

1
Case Western Reserve University and Case Medical Center, Cleveland, Ohio.
2
Veterans Administration Medical Center Research Service 151, Cleveland, Ohio.
3
These authors contributed equally to this work.

Abstract

Diabetic retinopathy is a major cause of visual impairment, which continues to increase in prevalence as more and more people develop diabetes. Despite the importance of vision, the retina is one of the smallest tissues in the body, and specialized techniques have been developed to study retinopathy. This article summarizes several methods used to (i) induce diabetes in mice, (ii) maintain the diabetic animals throughout the months required for development of typical vascular histopathology, (iii) evaluate vascular histopathology of diabetic retinopathy, and (iv) quantitate abnormalities implicated in the development of the retinopathy.

KEYWORDS:

diabetes; mouse; retina; retinopathy; streptozotocin

PMID:
26331759
PMCID:
PMC4597317
DOI:
10.1002/9780470942390.mo140190
[Indexed for MEDLINE]
Free PMC Article

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