Format

Send to

Choose Destination
Bonekey Rep. 2015 Aug 19;4:732. doi: 10.1038/bonekey.2015.101. eCollection 2015.

Assessment of muscle mass and strength in mice.

Author information

1
Department of Surgery, Indiana University School of Medicine , Indianapolis, IN, USA ; Indiana University Melvin and Bren Simon Cancer Center , Indianapolis, IN, USA.
2
Department of Medicine/Cardiology Unit, Karolinska Institute , Stockholm, Sweden ; Department of Physiology and Pharmacology, Karolinska Institute , Stockholm, Sweden.
3
Indiana University Melvin and Bren Simon Cancer Center , Indianapolis, IN, USA ; Department of Medicine, Indiana University School of Medicine , Indianapolis, IN, USA.

Abstract

Muscle weakness is an important phenotype of many diseases that is linked to impaired locomotion and increased mortality. The force that a muscle can generate is determined predominantly by muscle size, fiber type and the excitation-contraction coupling process. Here we describe methods for the histological assessment of whole muscle to determine fiber cross-sectional area and fiber type, determination of changes in myocyte size using C2C12 cells, in vivo functional tests and measurement of contractility in dissected whole muscles. The extensor digitorum longus and soleus muscles are ideally suited for whole-muscle contractility, and dissection of these muscles is described.

Supplemental Content

Full text links

Icon for Nature Publishing Group Icon for PubMed Central
Loading ...
Support Center