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Mol Imaging Biol. 2016 Apr;18(2):243-8. doi: 10.1007/s11307-015-0890-0.

A Comparison of microCT and microPET for Evaluating Lymph Node Metastasis in a Rat Model.

Author information

1
Department of Diagnostic and Interventional Radiology, University Hospital Heidelberg, Heidelberg, Germany.
2
Translational Lung Research Center Heidelberg, Member of the German Center for Lung Research DZL, Heidelberg, Germany.
3
Department of Nuclear Medicine, University Hospital Heidelberg, INF 400, 69120, Heidelberg, Germany.
4
Institute of Pathology, University Hospital Heidelberg, Heidelberg, Germany.
5
Core Facility Small Animal Imaging, German Cancer Research Center, Heidelberg, Germany.
6
Molecular Radiation Oncology, German Cancer Research Center and University Hospital Center Heidelberg, Heidelberg, Germany.
7
Cooperation Unit Nuclear Medicine, German Cancer Research Center (DKFZ), Heidelberg, Germany.
8
Department of Nuclear Medicine, University Hospital Heidelberg, INF 400, 69120, Heidelberg, Germany. frederik@egiesel.com.
9
Cooperation Unit Nuclear Medicine, German Cancer Research Center (DKFZ), Heidelberg, Germany. frederik@egiesel.com.

Abstract

PURPOSE:

The demand to optimize multidisciplinary treatment strategies in patients with benign and malignant diseases of the lung and other organs has led to the increased need of mechanistic proof-of-concept studies in preclinical small animal models using new non-invasive imaging methods. Therefore, we evaluated the role of microPET and microCT for mediastinal lymph node staging in an orthotopic lung cancer model in rats.

PROCEDURES:

Human lung cancer cells (NCI-H460) were injected transthoracically in nude rats (NIH-RNU). After 2 weeks of tumour growth, animals underwent multiphase contrast-enhanced microCT using ExiTron nano 12000 as a contrast agent and dynamic microPET using the tracer 2-deoxy-2-[(18)F]fluoro-D-glucose ([(18)F]FDG). Thereafter, animals were sacrificed for histological analysis.

RESULTS:

Late phase micro X-ray computed tomography (microCT) revealed the best delineation of lymph node metastases, as compared to earlier scans. In terms of an increased [(18)F]FDG uptake over time, dynamic micro positron emission tomography (microPET) delineated lymph node metastases and enabled metabolic examinations of the induced lung cancer metastases.

CONCLUSION:

The combination of contrast-enhanced microCT and dynamic microPET is feasible in rats for the visualization of mediastinal lymph node metastases.

KEYWORDS:

Computed tomography; Lung cancer; Orthotopic lung cancer model; [18F]FDG/PET-CT

PMID:
26330160
DOI:
10.1007/s11307-015-0890-0
[Indexed for MEDLINE]

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