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Mol Med Rep. 2015 Nov;12(5):7005-10. doi: 10.3892/mmr.2015.4253. Epub 2015 Aug 26.

Upregulation of microRNA-132 in gastric cancer promotes cell proliferation via retinoblastoma 1 targeting.

Author information

1
Department of Gastroenterology, Hiser Medical Group of Qingdao, Medical College of Qingdao University, Qingdao, Shandong 266033, P.R. China.
2
The Maternity Centre, The No. 8 People's Hospital of Qingdao, Qingdao, Shandong 266041, P.R. China.
3
Department of Rehabilitation, The Third People's Hospital of Qingdao, Qingdao, Shandong 266041, P.R. China.
4
Department of Gastroenterology, Qingdao Municipal Hospital, Medical College of Qingdao University, Qingdao, Shandong 266033, P.R. China.

Abstract

Gastric cancer is one of the most frequent malignancies and a leading cause of cancer-related mortality worldwide. MicroRNAs (miRs), a class of small non‑coding RNAs, have been shown to be critical in tumorigenesis. In the present study, the expression levels of miR‑132 were analyzed in gastric cancer samples using quantitative reverse transcription‑polymerase chain reaction. In addition, the cell viability, proliferation and invasion abilities were determined in two gastric cancer cell lines, NCI‑N87 and MGC80‑3, that were transfected with miR‑132 mimics or antisense oligos. It was found that miR‑132 expression was significantly upregulated in gastric cancer tissues when compared with adjacent non‑cancerous tissues. At the molecular level, the data demonstrated that miR‑132 inhibits the protein levels of retinoblastoma 1 (RB1) by targeting the 3'‑untranslated region. Furthermore, reintroduction of RB1 markedly attenuated the proliferative roles of miR‑132 overexpression. Therefore, the present results indicate that the miR‑132/RB1 regulatory axis may be a potential novel diagnostic and therapeutic target for the treatment of gastric cancer.

PMID:
26324336
DOI:
10.3892/mmr.2015.4253
[Indexed for MEDLINE]

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