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Nat Methods. 2015 Oct;12(10):955-8. doi: 10.1038/nmeth.3556. Epub 2015 Aug 31.

Inntags: small self-structured epitopes for innocuous protein tagging.

Author information

1
Molecular Biology Institute of Barcelona (IBMB), Consejo Superior de Investigaciones Científicas (CSIC), Barcelona, Spain.
2
Department of Microbiology and Immunology, School of Pharmacy, Zagazig University, Zagazig, Egypt.
3
Barcelona Supercomputing Center (BSC), Barcelona, Spain.
4
Joint BSC-CRG-IRB Programme in Computational Biology, Barcelona, Spain.
5
AbBcn Ltd, Bellaterra, Spain.
6
Immunostep Ltd, Salamanca, Spain.
7
Departament de Bioquimica, Facultat de Biologia, Universitat de Barcelona, Barcelona, Spain.
8
Institute for Research in Biomedicine (IRB Barcelona), Barcelona, Spain.

Abstract

Protein tagging is widely used in approaches ranging from affinity purification to fluorescence-based detection in live cells. However, an intrinsic limitation of tagging is that the native function of the protein may be compromised or even abolished by the presence of the tag. Here we describe and characterize a set of small, innocuous protein tags (inntags) that we anticipate will find application in a variety of biological techniques.

PMID:
26322837
DOI:
10.1038/nmeth.3556
[Indexed for MEDLINE]

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