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Nat Chem Biol. 2015 Oct;11(10):762-4. doi: 10.1038/nchembio.1896. Epub 2015 Aug 31.

Monobody-mediated alteration of enzyme specificity.

Author information

1
Department of Biochemistry and Molecular Biology, The University of Chicago, Chicago, Illinois, USA.
2
Frontier Research Department, Gifu R&D Center, Amano Enzyme, Inc., Gifu, Japan.

Abstract

Current methods for engineering enzymes modify enzymes themselves and require a detailed mechanistic understanding or a high-throughput assay. Here, we describe a new approach where catalytic properties are modulated with synthetic binding proteins, termed monobodies, directed to an unmodified enzyme. Using the example of a β-galactosidase from Bacillus circulans, we efficiently identified monobodies that restricted its substrates for its transgalactosylation reaction and selectively enhanced the production of small oligosaccharide prebiotics.

PMID:
26322825
PMCID:
PMC4989918
DOI:
10.1038/nchembio.1896
[Indexed for MEDLINE]
Free PMC Article
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