Format

Send to

Choose Destination
Curr Opin Biotechnol. 2015 Dec;36:115-21. doi: 10.1016/j.copbio.2015.08.007. Epub 2015 Aug 29.

Programmable genetic circuits for pathway engineering.

Author information

1
Energy, Environmental and Chemical Engineering, Washington University in St. Louis, St. Louis, MO 63130, USA.
2
Energy, Environmental and Chemical Engineering, Washington University in St. Louis, St. Louis, MO 63130, USA. Electronic address: tsmoon@wustl.edu.

Abstract

Synthetic biology has the potential to provide decisive advances in genetic control of metabolic pathways. However, there are several challenges that synthetic biologists must overcome before this vision becomes a reality. First, a library of diverse and well-characterized sensors, such as metabolite-sensing or condition-sensing promoters, must be constructed. Second, robust programmable circuits that link input conditions with a specific gene regulation response must be developed. Finally, multi-gene targeting strategies must be integrated with metabolically relevant sensors and complex, robust logic. Achievements in each of these areas, which employ the CRISPR/Cas system, in silico modeling, and dynamic sensor-regulators, among other tools, provide a strong basis for future research. Overall, the future for synthetic biology approaches in metabolic engineering holds immense promise.

PMID:
26322736
DOI:
10.1016/j.copbio.2015.08.007
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center