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J Biosci Bioeng. 2016 Mar;121(3):349-53. doi: 10.1016/j.jbiosc.2015.06.017. Epub 2015 Aug 28.

Extra-facile chiral separation of amino acid enantiomers by LC-TOFMS analysis.

Author information

1
Department of Biotechnology, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan.
2
Department of Biotechnology, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan. Electronic address: fukusaki@bio.eng.osaka-u.ac.jp.

Abstract

An extra-facile chiral liquid chromatography-time of flight mass spectrometry (LC-TOFMS) analytical method of amino acid enantiomers has been developed without a derivatization process. The enantioseparation of eighteen proteinogenic amino acids (except for proline) was simultaneously performed using a combination of a chiral column (CROWNPAK CR-I(+)) and a TOFMS within 15 min. An isocratic condition of a simple mobile phase comprising acetonitrile/water/trifluoroacetic acid (96/4/0.5) gave baseline separation of all underivatized amino acid enantiomers on the chiral column.

KEYWORDS:

Chiral liquid chromatography; Time of flight mass spectrometry; Underivatized amino acids; d-Amino acids

PMID:
26321292
DOI:
10.1016/j.jbiosc.2015.06.017
[Indexed for MEDLINE]

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