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J Extracell Vesicles. 2015 Aug 28;4:26533. doi: 10.3402/jev.v4.26533. eCollection 2015.

Meeting report: discussions and preliminary findings on extracellular RNA measurement methods from laboratories in the NIH Extracellular RNA Communication Consortium.

Author information

1
Division of Maternal Fetal Medicine, Department of Reproductive Medicine, University of California San Diego, San Diego, CA, USA.
2
Sanford Consortium for Regenerative Medicine, San Diego, CA, USA; llaurent@ucsd.edu.
3
Department of Urology, Tulane University, School of Medicine, New Orleans, LA, USA.
4
Phoenix Children's Hospital, Phoenix, AZ, USA.
5
Department of Neurology, Harvard Medical School, Massachusetts General Hospital, Boston, MA, USA.
6
Program in Neuroscience, Harvard Medical School, Massachusetts General Hospital, Boston, MA, USA.
7
Department of Radiology, Harvard Medical School, Massachusetts General Hospital, Boston, MA, USA.
8
Neurogenomics Division, Translational Genomics Research Institute, Phoenix, AZ, USA.
9
Center for Theoretical and Applied Neuro-Oncology, Division of Neurosurgery, University of California San Diego, San Diego, CA, USA.
10
School of Dentistry, University of California, Los Angeles, CA, USA.
11
Program in Cellular and Molecular Medicine, Boston Children's Hospital, Boston, MA, USA.
12
Cardiovascular Institute, Beth Israel Deaconess Medical Center, Boston, MA, USA.
13
Exosome Diagnostics GmbH, Martinsried, Germany.
14
NXPharmaGen, Miami, FL, USA.
15
Division of Preclinical Innovation, National Center for Advancing Translational Sciences (NCATS), National Institutes of Health, Bethesda, MD, USA.
16
Department of Medicine, University of Massachusetts Medical School, Worcester, MA, USA.
17
Pacific Northwest Diabetes Research Institute, Seattle, WA, USA.
18
Luxembourg Centre for Systems Biomedicine, University of Luxembourg, Esch-sur-Alzette, Luxembourg.
19
Department of Neurology, Center for Neurologic Diseases, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA.
20
TGen, Phoenix, AZ, USA.
21
Barrow Neurological Institute, Phoenix, AZ, USA.
22
Department of Neurology, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA.
23
Department of Pharmacology, Tulane University School of Medicine, New Orleans, LA, USA.
24
Department of Transplantation, Mayo Clinic Florida, Jacksonville, FL, USA.
25
Department of Cancer Biology, Mayo Clinic Florida, Jacksonville, FL, USA.
26
Department of Surgery, University of California San Francisco, San Francisco, CA, USA.
27
Department of Veteran's Affairs, San Francisco, CA, USA.
28
Center for Cancer Computational Biology, Dana Farber Cancer Institute, Boston, MA, USA.
29
Exosome Diagnostics, Cambridge, MA, USA.
30
Department of Molecular Physiology and Biophysics, Vanderbilt University Medical Center, Nashville, TN, USA.
31
Division of Cardiovascular Medicine, Department of Medicine, Vanderbilt University Medical Center, Nashville, TN, USA.
32
Department of Pathology, Medical College of Wisconsin, Milwaukee, WI, USA.
33
Department of Biological Chemistry, Johns Hopkins University, Baltimore, MD, USA.

Abstract

Extracellular RNAs (exRNAs) have been identified in all tested biofluids and have been associated with a variety of extracellular vesicles, ribonucleoprotein complexes and lipoprotein complexes. Much of the interest in exRNAs lies in the fact that they may serve as signalling molecules between cells, their potential to serve as biomarkers for prediction and diagnosis of disease and the possibility that exRNAs or the extracellular particles that carry them might be used for therapeutic purposes. Among the most significant bottlenecks to progress in this field is the lack of robust and standardized methods for collection and processing of biofluids, separation of different types of exRNA-containing particles and isolation and analysis of exRNAs. The Sample and Assay Standards Working Group of the Extracellular RNA Communication Consortium is a group of laboratories funded by the U.S. National Institutes of Health to develop such methods. In our first joint endeavour, we held a series of conference calls and in-person meetings to survey the methods used among our members, placed them in the context of the current literature and used our findings to identify areas in which the identification of robust methodologies would promote rapid advancements in the exRNA field.

KEYWORDS:

RNA sequencing; exosomes; extracellular RNA; extracellular vesicles; microvesicles

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