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Biomed Pharmacother. 2015 Oct;75:137-41. doi: 10.1016/j.biopha.2015.07.020. Epub 2015 Aug 28.

Down-regulation of MicroRNA-381 promotes cell proliferation and invasion in colon cancer through up-regulation of LRH-1.

Author information

1
The Third Department of Surgery, The Fourth Hospital of Hebei Medical University, Shijiazhuang, 050011, China.
2
The Third Department of Surgery, The Fourth Hospital of Hebei Medical University, Shijiazhuang, 050011, China. Electronic address: li_yong_hbth@126.com.

Abstract

The expression and roles of MicroRNA-381 (miR-381) has been explored in several types of human cancers. However, its biological functions in colon cancer remain largely unknown. Quantitative real-time PCR assays were used to detect the expression of miR-381 in human colon cancer tissues and adjacent normal tissues. miR-381 antisense oligos and mimics were introduced into SW480 and HCT116 cells. Bioinformatic prediction analysis was performed to identify the potential targets of miR-381. Protein expression analysis, luciferase assays and rescue assays were used to confirm the substrate of miR-381. We found that miR-381 was significantly down-regulated in human colon cancer tissues, compared with adjacent normal tissues. Introduction of miR-381 antisense oligos into SW480 and HCT116 cells promoted cell proliferation and invasion. Besides, inhibition of miR-381 could also support tumor growth in the nude mice. Additionally, bioinformatic prediction suggested that the nuclear receptor liver receptor homologue 1 (LRH-1) is a target gene of miR-381. Thus, our data suggested that down-regulation of miR-381 plays an important role in the colon cancer progression.

KEYWORDS:

Colon cancer; LRH-1; MicroRNA; miR-381

PMID:
26320367
DOI:
10.1016/j.biopha.2015.07.020
[Indexed for MEDLINE]

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