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J Pharm Biomed Anal. 2015 Nov 10;115:570-5. doi: 10.1016/j.jpba.2015.08.014. Epub 2015 Aug 18.

Metabolism study of boldenone in human urine by gas chromatography-tandem mass spectrometry.

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Kunming Medical University, Yunnan 650500, China.
Beijing University of Chinese Medicine, Beijing 100029, China.
Beijing University of Chinese Medicine, Beijing 100029, China. Electronic address:


Boldenone (BOLD), an anabolic steroid, is likely to be abused in livestock breeding and in sports. Although some of BOLD metabolites in human urine, such as 5β-adrost-1-en-17β-ol-3-one (BM1), have been detected, investigations on their excretion patterns for both genders are insufficient. Moreover, little research on 17α-BOLD glucuronide as a metabolite in human urine has been reported. The aim of this study is to make a contribution to the knowledge of 17β-BOLD metabolism in humans. Three male and three female volunteers were orally administrated with 30mg 17β-BOLD. Urine samples were collected and analyzed with gas chromatography-tandem mass spectrometry. The data proved that 17β-BOLD, BM1, and 17α-BOLD were excreted in urine in both free and glucuronic conjugated forms after administration of 17β-BOLD. For most subjects, the urinary concentrations of BM1 were higher than that of 17β-BOLD. 17α-BOLD was excreted in small amounts. 17α-BOLD, 17β-BOLD, and BM1 were present naturally in urine with low concentrations. Administration of 30mg 17β-BOLD could not influence the excretion profiles of urinary androsterone, etiocholanolone, and testosterone/epitestosterone ratio. There were no differences in BOLD metabolic patterns between man and woman.


Boldenone; Gas chromatography–tandem mass spectrometry; Metabolism

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