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Lung. 2015 Dec;193(6):947-53. doi: 10.1007/s00408-015-9797-4. Epub 2015 Aug 30.

Morphological and Functional Alterations of Alveolar Macrophages in a Murine Model of Chronic Inflammatory Lung Disease.

Author information

1
Infection Immunology Group, Institute of Medical Microbiology and Hospital Hygiene, Infection Control and Prevention, Otto-von-Guericke University, Leipziger Straße 44, 39120, Magdeburg, Germany. julia.boehme@helmholtz-hzi.de.
2
Immune Regulation Group, Helmholtz Centre for Infection Research, Inhoffenstraße 7, 38124, Brunswick, Germany. julia.boehme@helmholtz-hzi.de.
3
Department of Translational Inflammation Research, Institute of Experimental Internal Medicine, Otto-von-Guericke University, Pfälzer Platz, Gebäude 28, 39106, Magdeburg, Germany.
4
Infection Immunology Group, Institute of Medical Microbiology and Hospital Hygiene, Infection Control and Prevention, Otto-von-Guericke University, Leipziger Straße 44, 39120, Magdeburg, Germany.
5
Immune Regulation Group, Helmholtz Centre for Infection Research, Inhoffenstraße 7, 38124, Brunswick, Germany.

Abstract

PURPOSE:

Chronic lung inflammation commonly induces a multitude of structural and functional adaptations within the lung tissue and airspaces. Yet the impact of a persistent inflammatory environment on alveolar macrophages is still incompletely understood. Here, we examined morphology and function of alveolar macrophages in a transgenic mouse model of chronic lung disease.

METHODS:

Imaging flow cytometry, flow cytometry, and microscopic evaluation of alveolar macrophages isolated from healthy and inflamed lungs were performed. Gene expression of polarization markers was compared by quantitative real-time RT-PCR. The pro-inflammatory immune response of alveolar macrophages toward bacterial ligands was assessed in in vivo clodronate-liposome depletion studies.

RESULTS:

Chronic lung inflammation is associated with a substantially altered, activated alveolar macrophage morphology, and blunted TNF-α response by these cells following stimulation with ligands derived from the respiratory pathogen Streptococcus pneumoniae.

CONCLUSIONS:

These results demonstrate pleiotropic effects of pulmonary inflammation on alveolar macrophage phenotype and function and suggest a functional impairment of these cells during infection with airborne pathogens.

KEYWORDS:

Alveolar macrophage; Chronic lung disease; Infection; Inflammation; Macrophage polarization

PMID:
26319657
PMCID:
PMC4651980
DOI:
10.1007/s00408-015-9797-4
[Indexed for MEDLINE]
Free PMC Article

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