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J Inorg Biochem. 2015 Nov;152:186-9. doi: 10.1016/j.jinorgbio.2015.08.003. Epub 2015 Aug 15.

Effects of aluminium chloride and aluminium chlorohydrate on DNA repair in MCF10A immortalised non-transformed human breast epithelial cells.

Author information

1
School of Biological Sciences, University of Reading, Reading RG6 6UB, UK.
2
School of Biological Sciences, University of Reading, Reading RG6 6UB, UK. Electronic address: p.d.darbre@reading.ac.uk.

Abstract

Use of underarm aluminium (Al)-based antiperspirant salts may be a contributory factor in breast cancer development. At the 10th Keele meeting, Al was reported to cause anchorage-independent growth and double strand DNA breaks in MCF10A immortalised non-transformed human breast epithelial cells. We now report that exposure of MCF10A cells to Al chloride or Al chlorohydrate also compromised DNA repair systems. Long-term (19-21 weeks) exposure to Al chloride or Al chlorohydrate at a 10(-4) M concentration resulted in reduced levels of BRCA1 mRNA as determined by real-time RT-PCR and BRCA1 protein as determined by Western immunoblotting. Reduced levels of mRNA for other DNA repair genes (BRCA2, CHK1, CHK2, Rad51, ATR) were also observed using real-time RT-PCR. Loss of BRCA1 or BRCA2 gene function has long been associated with inherited susceptibility to breast cancer but these results suggest that exposure to aluminium-based antiperspirant salts may also reduce levels of these key components of DNA repair in breast epithelial cells. If Al can not only damage DNA but also compromise DNA repair systems, then there is the potential for Al to impact on breast carcinogenesis.

KEYWORDS:

Aluminium chloride; Aluminium chlorohydrate; Antiperspirant; BRCA1; BRCA2; Breast cancer; DNA repair; MCF10A cells

PMID:
26319584
DOI:
10.1016/j.jinorgbio.2015.08.003
[Indexed for MEDLINE]

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