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Appl Biochem Biotechnol. 2015 Nov;177(6):1327-37. doi: 10.1007/s12010-015-1817-6. Epub 2015 Aug 29.

Clenbuterol Assay by Spectral Imaging Surface Plasmon Resonance Biosensor System.

Author information

1
Functional Materials Research Laboratory, Tongji University, Shanghai, People's Republic of China.
2
School of Science, Xi'an Jiaotong University, Xi'an, People's Republic of China. xiangyifang@sina.com.
3
Department of Clinical Laboratory, the First Affiliated Hospital of Medical College, Xi'an Jiaotong University, Xi'an, People's Republic of China.

Abstract

To prevent illegal use of clenbuterol and for quality control in the food industry, more efficient and reliable methods for clenbuterol detection are needed. In this study, clenbuterol was detected using a spectral imaging surface plasmon resonance sensor system via two inhibition methods: (1) the target site compensation method, in which anti-clenbuterol antibody was immobilized on the sensor chip as a bioprobe and (2) the solution competition method in which a clenbuterol-BSA conjugate was immobilized on the sensor chip as the bioprobe. The detectable clenbuterol concentration ranged between 6.25 and 100 μg/mL for both methods. The clenbuterol limit of detection for the target site compensation method and solution competition method are estimated to be 6.7 and 4.5 μg/mL, respectively. The proposed methods were successfully applied to the detection of clenbuterol molecules and were found to have high specificity and high-throughput and were label free and operationally convenient.

KEYWORDS:

Food safety; Low-molecular-weight hapten detection; Multichannel measurement; Surface plasmon resonance biosensor

PMID:
26319570
DOI:
10.1007/s12010-015-1817-6
[Indexed for MEDLINE]

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