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Phytochemistry. 2015 Oct;118:181-91. doi: 10.1016/j.phytochem.2015.08.008. Epub 2015 Aug 25.

Metabolite profiling and expression analysis of flavonoid, vitamin C and tocopherol biosynthesis genes in the antioxidant-rich sea buckthorn (Hippophae rhamnoides L.).

Author information

1
Department of Biology, University of Western Ontario, London, ON, Canada. Electronic address: tfatima@uwo.ca.
2
Department of Biology, University of Western Ontario, London, ON, Canada. Electronic address: vigyakesari@gmail.com.
3
Department of Biology, University of Western Ontario, London, ON, Canada. Electronic address: iwatt@uwo.ca.
4
Departments of Computing Science and Biological Sciences, University of Alberta, Edmonton, AB, Canada. Electronic address: david.wishart@ualberta.ca.
5
Ontario Ministry of Agriculture, Food and Rural Affairs, Simcoe Resource Centre, Simcoe, ON, Canada. Electronic address: jim.todd@ontario.ca.
6
Agroforestry Development Centre, Agriculture and Agri-Food Canada, Indian Head, SK, Canada. Electronic address: Bill.Schroeder@AGR.GC.CA.
7
Department of Plant Agriculture, University of Guelph, ON, Canada. Electronic address: gpaliyat@uofguelph.ca.
8
Department of Biology, University of Western Ontario, London, ON, Canada; School of Environmental & Rural Sciences, University of New England, Armidale, NSW, Australia. Electronic address: pkrishna@uwo.ca.

Abstract

In this study, phenolic compounds were analyzed in developing berries of four Canadian grown sea buckthorn (Hippophae rhamnoides L.) cultivars ('RC-4', 'E6590', 'Chuyskaya' and 'Golden Rain') and in leaves of two of these cultivars. Among phenolic acids, p-coumaric acid was the highest in berries, while gallic acid was predominant in leaves. In the flavonoid class of compounds, myricetin/rutin, kaempferol, quercetin and isorhamnetin were detected in berries and leaves. Berries of the 'RC-4' cultivar had approximately ⩾ 2-fold higher levels of myricetin and quercetin at 17.5mg and 17.2 mg/100 g FW, respectively, than the other cultivars. The flavonoid content in leaves was considerably more than in berries with rutin and quercetin levels up to 135 mg and 105 mg/100 g FW, respectively. Orthologs of 15 flavonoid biosynthesis pathway genes were identified within the transcriptome of sea buckthorn mature seeds. Semi-quantitative RT-PCR analysis of these genes in developing berries indicated relatively higher expression of genes such as CHS, F3'H, DFR and LDOX in the 'RC-4' cultivar than in the 'Chuyskaya' cultivar. Vitamin C levels in ripened berries of the Canadian cultivars were on the high end of the concentration range reported for most other sea buckthorn cultivars. Orthologs of genes involved in vitamins C and E biosynthesis were also identified, expanding the genomic resources for this nutritionally important plant.

KEYWORDS:

Elaeagnaceae; Flavonoids; Gene expression; Hippophae rhamnoides L.; Phenolic acids; Sea buckthorn; Tocopherol; Transcriptome; Vitamin C

PMID:
26318327
DOI:
10.1016/j.phytochem.2015.08.008
[Indexed for MEDLINE]

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