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Int J Biol Macromol. 2015 Nov;81:656-61. doi: 10.1016/j.ijbiomac.2015.08.043. Epub 2015 Aug 24.

A polysaccharide from cultured mycelium of Hericium erinaceus and its anti-chronic atrophic gastritis activity.

Author information

1
Affiliated Hospital, Changchun University of Chinese Medicine, Changchun 130117, China; International Collaborative Research Center, Changchun University of Chinese Medicine, Changchun 130117, China.
2
Research and Development Center, Changchun University of Chinese Medicine, Changchun 130117, China.
3
International Collaborative Research Center, Changchun University of Chinese Medicine, Changchun 130117, China; Research and Development Center, Changchun University of Chinese Medicine, Changchun 130117, China. Electronic address: gaoqipin@sina.com.

Abstract

A polysaccharide named EP-1 was found by screening cultured mycelium of Hericium erinaceus, which was extracted and subjected to precipitation with ethanol, hollow-fiber ultrafiltration and ion-exchange chromatography. The polysaccharide has a molecular weight of approximately 3100Da and is composed of glucose, mannose and galactose, thus being a heteroglycan. EP-1 has a backbone of α-d-Glc(1→3) and β-d-Glc(1→3). The β-d-Glc(1→3) and α-d-Gal-(1→3) were regarded as branches attached to the C-4 position. The α-d-Man was regarded as a terminal residue. The anti-CAG activity was evaluated in experimental systems using a cell model for identification. The polysaccharide significantly inhibited the growth of MC cells obtained from human gastric mucosa epithelium (GES-1) cells transformed by MNNG, which were used as a chronic atrophic gastritis cell model. It also interfered with the MC cells by inducing cell cycle arrest. Thus, EP-1 shows potential for the development of new functional foods and drugs.

KEYWORDS:

Anti-CAG activity; Hericium erinaceus; Polysaccharide

PMID:
26314904
DOI:
10.1016/j.ijbiomac.2015.08.043
[Indexed for MEDLINE]

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